Sanger sequencing IOS application from Thermo Fisher Scientific. A handy tool for new or experienced users who perform automated DNA sequencing using Capillary Electrophoresis. This application’s main feature is a chemistry guide which covers all aspects of Sanger sequencing from the basic principles to workflows/ applications. Main features include:
1) DNA Sequencing by Capillary Electrophoresis, Applied Biosystems Chemistry Guide, 3rd edition (2016). Chapters include:
o Sanger sequencing applications
o DNA template preparation
o Cycle sequencing
o Purification of extension products
o Capillary electrophoresis
o Data analysis
o Troubleshooting
2) How-to videos on every step of the Sanger sequencing workflow.
3) Popular videos from the Seq It Out, an educational video series that provides bite-sized answers to everyday sequencing questions.
4) Most recent application notes on Sanger sequencing.
5) Calculator for formulas referenced in the chemistry guide.
6) Direct link to Behind the Bench blog for the latest news, stories and updates on Sanger sequencing.